Peptides containing disulfide bridges
For peptides comprising several cysteines it is always difficult to ensure the correct disulfide ligation.
Using a number of different cysteine protecting groups during synthesis plus decades of knowhow in correct folding conditions for bridged peptides, PANATecs is capable of synthesising peptides containing complex bridges.
An application would be for example using peptides containing disulfide bridges/bonds as reference substances in mass spectrometry-based quality control of biologics or biosimilars.
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Peptide-Protein Conjugates
Peptide-protein conjugates are used in order to generate specific antibodies against peptide sequences.
Typically, individual peptide sequences are too small to trigger an adequate immune response. Therefore, they are linked covalently to bigger and more complex molecules (normally proteins), so that they show immunogenetic characteristics.
The epitopes contained in the carrier proteins, such as KLH (keyhole limpet hemocyanin), BSA (bovine serum albumin) and OVA (ovalbumin), stimulate the T helper cells. This supports triggering of an immune response of the B cells, inducing the biosynthesis of sequence-specific antibodies.
Since the immune system reacts to epitopes of the carrier protein as a whole, antibodies are generated against the carrier protein as well as against the peptide sequence.
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MelaCheck – T-cell monitoring for malignant melanoma
The peptide pools consist of overlapping peptide sequences of known melanoma tumor antigens. These synthetic peptides (15-mers) cover the entire amino acid sequence of the individual tumor antigens.
Available both as peptide pools and as individual peptides, the amino acid sequences are suitable for detecting tumorspecific human T cells in blood (e.g., from the PBMC fraction of an ELISPOT assay).
MelaCheck pools and individual peptides are geared toward a variety of potential customers, ranging from development professionals and research groups to those interested in the clinical applications of this tool in vaccination studies. In addition to its use as a positive control after immunization with tumor antigens, MelaCheck can also be used for general detection of the specific T-cell response in melanoma patients.
You will find Tumor Anitgens in our Product Shop:
Tumore Antigens (MAGE, MART, Pmel17, PRAME/OIP4)
P-CHECK Kit for reliable protease detection
Because proteases can cause serious problems, both in the raw materials used in the diagnostics industry and in the production of biopharmaceuticals, manufacturers should identify their presence early on. Test systems that are currently available on the market are often only capable of verifying individual protease classes.
P-CHECK, however, is a fluorescence-based system with a marked, complete peptide library of more than 2 million sequence variants, making it a universal, reliable and sensitive tool for detecting proteases.
Advantages of P-CHECK Kit:
• Rapid test in 96-well plate format
• Universal detection of unknown proteases
• High sensitivity in the low ng range
• Robust toward matrix influences
• Suitable for unprepared samples
You will find the original PANATecs P-CHECK Kit in our Product Shop.
CEF(T) control peptides for T-cell assays
The peptide pools contain HLA class I or II restricted epitopes from human cytomegalovirus (HCMV), Epstein-Barr virus (EBV), influenza virus (FLU) and/or from tetanus toxin. Most people have already been exposed to these antigens and have the corresponding CD8+ and CD4+ memory T cells. This means that, unlike traditional reagents such as PHA, PMA, or ionomycin, CEF and CEFT pools are ideally suited for use as specific positive controls for all T-cell-based assays (such as intracellular flow cytometry analysis or ELISPOT assays).
You will find Standard Positive Monitoring Peptide Pools in our Product Shop:
