N-terminal sequencing via Edman Degradation
You need to test whether the N-terminus of your proteins has been properly expressed? Or you want to know which cleavage site was created by a protease?
Send us your samples for fast and reliable testing via Edman Degradation (5 or 10 amino acids). Other numbers of Degradation cycles available on request.
Our offer:
- Rapid processing of samples at attractive prices
- N-terminal sequencing (5 or 10 amino acids)
- Result sheet as pdf via email, see example
Please send us:
- Your pure protein or your antibody in solution with adequate purity, blotted in to a PVDF membrane, in solution or as lyophilisate in a 1.5ml Eppendorf container1
- Please mark the side of the membrane on which the sample has been blotted, using a pencil cross in one corner
- Your signed order form
Please note that in the event of inadequate sample conditions (eg. blockage of the N-terminus, insufficient quantity) sequencing will not be possible, and a processing charge of 360 € will be charged.
1For gel bands/spots which are not delivered to us in 1.5ml Eppendorf containers, we need to add an amount of 5.00 € per sample for additional labour.